Part:BBa_K3661005:Design

YebF-Hiracin JM79-flag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 108
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 424
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

In order to improve the transcription and translation efficiency of bacteriocins, we have optimized their sequence.

Source

YebF:Escherichia coli strain BL21(DE3) genome, sequence found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/EF648003.1 Hiracin JM79:Enterococcus hirae genome, sequence found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/DQ664500.1 flag: sequence found through doi:10.1038/nbt1088-1204